Journal: Journal of Antimicrobial Chemotherapy

Article Title: The tissue distribution and pharmacokinetics of [ 14 C]-olorofim following intravenous dosing in the rat

doi: 10.1093/jac/dkag120

Figure Lengend Snippet: Structure of [ 14 C] -o lorofim. Olorofim was labelled at two positions in case of cleavage across the amide link.

Article Snippet: Radiolabelled olorofim (molecular weight of 500.18) was synthesized by Selcia Ltd; as the stability of the amide link was not then certain, the drug was labelled with [ 14 C] in two positions in case of cleavage across the amide link (Figure ).

Techniques:

Journal: Journal of Antimicrobial Chemotherapy

Article Title: The tissue distribution and pharmacokinetics of [ 14 C]-olorofim following intravenous dosing in the rat

doi: 10.1093/jac/dkag120

Figure Lengend Snippet: Distribution of radioactivity in the albino male rat: 2.25 h post-start of infusion of [ 14 C]-olorofim (three different sagittal sections shown).

Article Snippet: Radiolabelled olorofim (molecular weight of 500.18) was synthesized by Selcia Ltd; as the stability of the amide link was not then certain, the drug was labelled with [ 14 C] in two positions in case of cleavage across the amide link (Figure ).

Techniques: Radioactivity

Journal: Journal of Antimicrobial Chemotherapy

Article Title: The tissue distribution and pharmacokinetics of [ 14 C]-olorofim following intravenous dosing in the rat

doi: 10.1093/jac/dkag120

Figure Lengend Snippet: Distribution of radioactivity in the male albino, partially pigmented male and female rats: 8 h post-start of infusion of [ 14 C]-olorofim. (a) Section through a male albino rat. (b) Section through a male partially pigmented rat. (c) Section through a female rat.

Article Snippet: Radiolabelled olorofim (molecular weight of 500.18) was synthesized by Selcia Ltd; as the stability of the amide link was not then certain, the drug was labelled with [ 14 C] in two positions in case of cleavage across the amide link (Figure ).

Techniques: Radioactivity

Journal: Journal of Antimicrobial Chemotherapy

Article Title: The tissue distribution and pharmacokinetics of [ 14 C]-olorofim following intravenous dosing in the rat

doi: 10.1093/jac/dkag120

Figure Lengend Snippet: Distribution of radioactivity in the albino male rat: 24 h post-start of infusion of [ 14 C]-olorofim (three different sagittal sections shown).

Article Snippet: Radiolabelled olorofim (molecular weight of 500.18) was synthesized by Selcia Ltd; as the stability of the amide link was not then certain, the drug was labelled with [ 14 C] in two positions in case of cleavage across the amide link (Figure ).

Techniques: Radioactivity

Journal: Journal of Antimicrobial Chemotherapy

Article Title: The tissue distribution and pharmacokinetics of [ 14 C]-olorofim following intravenous dosing in the rat

doi: 10.1093/jac/dkag120

Figure Lengend Snippet: Distribution of radioactivity in the albino male and female rats: 72 h post-start of infusion of [ 14 C]-olorofim. (a) Section through a male rat. (b) Section through a female rat.

Article Snippet: Radiolabelled olorofim (molecular weight of 500.18) was synthesized by Selcia Ltd; as the stability of the amide link was not then certain, the drug was labelled with [ 14 C] in two positions in case of cleavage across the amide link (Figure ).

Techniques: Radioactivity

Journal: Journal of Antimicrobial Chemotherapy

Article Title: The tissue distribution and pharmacokinetics of [ 14 C]-olorofim following intravenous dosing in the rat

doi: 10.1093/jac/dkag120

Figure Lengend Snippet: Distribution of radioactivity in the albino male and female rat 168 h post-start of infusion of [ 14 C]-olorofim. (a) Section through a male rat. (b) Section through a female rat.

Article Snippet: Radiolabelled olorofim (molecular weight of 500.18) was synthesized by Selcia Ltd; as the stability of the amide link was not then certain, the drug was labelled with [ 14 C] in two positions in case of cleavage across the amide link (Figure ).

Techniques: Radioactivity

Journal: Journal of Antimicrobial Chemotherapy

Article Title: The tissue distribution and pharmacokinetics of [ 14 C]-olorofim following intravenous dosing in the rat

doi: 10.1093/jac/dkag120

Figure Lengend Snippet: Maximum radioactivity tissue:plasma ratios (where ratio is >3) following a single IV infusion of [ 14 C]-olorofim to albino rats (over 0–24 h post-dose).

Article Snippet: Radiolabelled olorofim (molecular weight of 500.18) was synthesized by Selcia Ltd; as the stability of the amide link was not then certain, the drug was labelled with [ 14 C] in two positions in case of cleavage across the amide link (Figure ).

Techniques: Radioactivity, Clinical Proteomics

Journal: Journal of Antimicrobial Chemotherapy

Article Title: The tissue distribution and pharmacokinetics of [ 14 C]-olorofim following intravenous dosing in the rat

doi: 10.1093/jac/dkag120

Figure Lengend Snippet: Plasma concentration: time profile of radioactivity and olorofim in male and female rats following a 2 h IV infusion of [ 14 C]-olorofim.

Article Snippet: Radiolabelled olorofim (molecular weight of 500.18) was synthesized by Selcia Ltd; as the stability of the amide link was not then certain, the drug was labelled with [ 14 C] in two positions in case of cleavage across the amide link (Figure ).

Techniques: Clinical Proteomics, Concentration Assay, Radioactivity

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A novel 18 F-labelled tetrazine ester prosthetic group for improved radiolabelling and in vivo stability of proteins and peptides

doi: 10.1186/s41181-026-00430-6

Figure Lengend Snippet: Structures of [ 18 F]TzAm ( 1 ), [ 18 F]TzE ( 2 ) and [ 18 F]TzE2 ( 3 ), three compounds designed for IEDDA reaction with TCO-conjugated biomolecules

Article Snippet: These results clearly indicate that the [ 18 F]TzE2 radiolabelling strategy did not impair the binding capacity of the Z09591 Affibody molecule.

Techniques:

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A novel 18 F-labelled tetrazine ester prosthetic group for improved radiolabelling and in vivo stability of proteins and peptides

doi: 10.1186/s41181-026-00430-6

Figure Lengend Snippet: Synthesis of [ 18 F]TzE2

Article Snippet: These results clearly indicate that the [ 18 F]TzE2 radiolabelling strategy did not impair the binding capacity of the Z09591 Affibody molecule.

Techniques:

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A novel 18 F-labelled tetrazine ester prosthetic group for improved radiolabelling and in vivo stability of proteins and peptides

doi: 10.1186/s41181-026-00430-6

Figure Lengend Snippet: Stability of [ 18 F]TzE2-Z09591, [ 18 F]TzAm-Z09591 and [ 18 F]TzE-Z09591 in PBS formulation over time (shelflife) ( A ) and after 90 min incubation in human plasma ( B ). One star (*) indicates p < 0.05, two stars (**) indicate p < 0.01 and three stars (***) indicate p < 0.001 using ANOVA for comparison between groups

Article Snippet: These results clearly indicate that the [ 18 F]TzE2 radiolabelling strategy did not impair the binding capacity of the Z09591 Affibody molecule.

Techniques: Formulation, Incubation, Clinical Proteomics, Comparison

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A novel 18 F-labelled tetrazine ester prosthetic group for improved radiolabelling and in vivo stability of proteins and peptides

doi: 10.1186/s41181-026-00430-6

Figure Lengend Snippet: Binding of [ 18 F]TzE2-Z09591, [ 18 F]TzAm-Z09591 and [ 18 F]TzE-Z09591 to frozen sections of PDGFRβ overexpressing U87 xenografts by an in vitro autoradiography assay. Quantification of binding signal as percentage of total binding ( A ). Representative autoradiograms for each of the tracers, both total binding (5 nM, top row panels), and after pre-blocking with 2 µM unlabeled Z09591 (bottom row panels) ( B )

Article Snippet: These results clearly indicate that the [ 18 F]TzE2 radiolabelling strategy did not impair the binding capacity of the Z09591 Affibody molecule.

Techniques: Binding Assay, In Vitro, Autoradiography, Blocking Assay

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: A novel 18 F-labelled tetrazine ester prosthetic group for improved radiolabelling and in vivo stability of proteins and peptides

doi: 10.1186/s41181-026-00430-6

Figure Lengend Snippet: In vivo biodistribution over time of [ 18 F]TzE2-Z09591 ( A ), [ 18 F]TzAm-Z09591 ( B ) and [ 18 F]TzE-Z09591 ( C ) in female wt Balb/c mice. Biodistribution was assessed by post-mortem organ distribution measurement by gamma counter, for up to 120 min after intravenous administration of each tracer. A direct comparison with more repeats (n = 4–6) in immunodeficient nu/nu Balb/c mice was compared at 60 min post-injection ( D ). A star (*) indicates difference compared to uptake of [ 18 F]TzE2-Z09591

Article Snippet: These results clearly indicate that the [ 18 F]TzE2 radiolabelling strategy did not impair the binding capacity of the Z09591 Affibody molecule.

Techniques: In Vivo, Comparison, Injection